Journal: Journal of nutritional science and vitaminology

Article Title: Oral Supplementation of Tocotrienol-Rich Fraction Alleviates Severity of Ulcerative Colitis in Mice.

doi: 10.3177/jnsv.65.318

Figure Lengend Snippet: Fig. 5. Levels of malondialdehyde (MDA), a lipid per- oxidation biomarker, were assessed by ELISA assay in colonic tissue homogenates of mice receiving either vehicle, 75 mg/kg/d 5-ASA, 150 mg/kg/d TRF or 150 mg/kg/d aTP. Data are expressed as mean6stan- dard deviation. **** p,0.0001 vs DSS/Control group, assessed by one-way ANOVA, followed by Bonferroni’s post hoc test.

Article Snippet: Phospho-NF-kB p65 (Ser536) levels were determined using PathScan® ELISA kit (Cell Signalling Technology, Danvers, MA).

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Control

Journal: The British journal of nutrition

Article Title: Benefits of whole ginger extract in prostate cancer

doi: 10.1017/S0007114511003308

Figure Lengend Snippet: Ginger extract (GE) induces mitochondrially mediated intrinsic apoptosis. (A) Immunoblot analyses for BAX, Bcl2, cytoplasmic cytochrome c (Cyt c), cleaved caspase-3 and poly(ADP-ribose)polymerase (PARP). β-Actin was used as a loading control. (B) Quantification of the time-dependent increase in caspase-3 (Casp-3) activity on GE treatment. Cells were treated with GE for 0, 12, 24 and 48 h, and caspase-3 activity was analysed using the fluorogenic substrate Ac-DEVD-7-amino-4-trifluoromethyl-coumarin. Values are means of three independent experiments performed in triplicate, with standard deviations represented by vertical bars (P<0·05). Immunofluoresence micrographs of control and 250 μg/ml of GE-treated cells stained for cleaved (Ci) caspase-3 and (Di) PARP. (Cii, Dii) Quantification of activated caspase-3-positive and cleaved PARP-positive cells. (A colour version of this figure can be found online at www.journals.cambridge.org/bjn).

Article Snippet: Cyclin D1, cdk4, p-Rb, Bcl2, cytochrome c , cleaved caspase-3 and cleaved poly (ADP-ribose)polymerase (PARP) were from Cell Signaling (Beverly, MA, USA), Ki67 was from Zymed (South San Francisco, CA, USA) and β-actin was from Sigma (St Louis, MO, USA).

Techniques: Western Blot, Activity Assay, Staining

Journal: The British journal of nutrition

Article Title: Benefits of whole ginger extract in prostate cancer

doi: 10.1017/S0007114511003308

Figure Lengend Snippet: Ginger extract (GE) caused in vivo inhibition of tumour growth in human PC-3 xenografts on dietary feeding of GE. (A) Progression profile of tumour growth in control vehicle-treated ( ) and GE-treated ( ) mice at the time of treatment. (B) GE treatment was well tolerated, and the body weights of the control ( ) and GE-treated ( ) groups were comparable. Values are means, with standard deviations represented by vertical bars (n = 6, P < 0·05). (C) Tumour micrographs from control and GE-treated mice, respectively, at 100× and 200× magnification. GE-treated tumour microsections reveal large areas of tumour cell death, consistent with the therapeutic effects of GE. Microsections from control tumour tissue show sheets of tumour cells with high-grade pleomorphic nuclei with minimal cell death. (D) Western blot analysis of tumour tissue lysates from control and GE-treated mice for cyclin B, cyclin D1, cyclin E, p21 and cleaved caspase-3. (A colour version of this figure can be found online at www.journals.cambridge.org/bjn).

Article Snippet: Cyclin D1, cdk4, p-Rb, Bcl2, cytochrome c , cleaved caspase-3 and cleaved poly (ADP-ribose)polymerase (PARP) were from Cell Signaling (Beverly, MA, USA), Ki67 was from Zymed (South San Francisco, CA, USA) and β-actin was from Sigma (St Louis, MO, USA).

Techniques: In Vivo, Inhibition, Western Blot

Journal: The British journal of nutrition

Article Title: Benefits of whole ginger extract in prostate cancer

doi: 10.1017/S0007114511003308

Figure Lengend Snippet: (A) Immunohistochemical staining of paraffin-embedded tumour tissue sections from the control and ginger extract (GE)-treated groups for proliferation marker (Ki67) and apoptotic markers (cleaved caspase-3 (casp-3), cleaved poly(ADP-ribose)polymerase (PARP) and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL)). (B) Quantification of Ki67, cleaved casp-3, cleaved PARP and TUNEL-positive cells counted from several randomly selected fields for a total of 200 cells. Values are means, with standard deviations represented by vertical bars (P < 0·05). Control, ; GE, . (A colour version of this figure can be found online at www.journals.cambridge.org/bjn).

Article Snippet: Cyclin D1, cdk4, p-Rb, Bcl2, cytochrome c , cleaved caspase-3 and cleaved poly (ADP-ribose)polymerase (PARP) were from Cell Signaling (Beverly, MA, USA), Ki67 was from Zymed (South San Francisco, CA, USA) and β-actin was from Sigma (St Louis, MO, USA).

Techniques: Immunohistochemical staining, Staining, Marker, TUNEL Assay